For the most up-to-date list of publications, please see my Google Scholar profile.
2025
Lucas Dada, Emiko Nagai, Sashank Agrawal, Ariel S. Wirchnianski, Ian A. Wilson, Kartik Chandran, Seiya Kitamura*
Frontiers in Immunology 2025, https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1533037/full
tags: High-throughput medicinal chemistry, infectious disease, SuFEx, inhibition
Alexandra Schurer, Humaira Ilyas, Maxim I. Maron, Subray Hegde, Melissa R. Leyden, Irene Roy, Rotila Hyka, Lucas Dada, Jeffrey Shabanowitz, Donald F. Hunt, Ellen Angeles, Victor Morell, Benjamin M. Lorton, Shira Glushakow-Smith, Daniel K. Borger, Yuxuan Wang, Linde Miles, Roger Belizaire, Seiya Kitamura, Kira Gritsman, David Shechter
bioRxiv 2025, https://www.biorxiv.org/content/10.1101/2025.04.07.647605v1.full
tags: cancer, inhibition, screening
2024
In vivo–Active Soluble Epoxide Hydrolase–targeting PROTACs with Improved Potency and Stability
Keita Nakane, Christophe Morisseau, Presley D. Dowker-Key, Gabrielle Benitez, Jennifer T. Aguilan, Emiko Nagai, Simone Sidoli, Bruce D. Hammock, Ahmed Bettaieb, Kosaku Shinoda, Seiya Kitamura*
ACS Medicinal Chemistry Letters 2024, https://pubs.acs.org/doi/10.1021/acsmedchemlett.4c00357
bioRxiv (preprint) 2024, https://www.biorxiv.org/content/10.1101/2024.07.23.604814v1
tags: PROTACs, soluble epoxide hydrolase, ER stress
Insights into the Interaction Landscape of the EVH1 Domain of Mena
Lanette LaComb, Agnidipta Ghosh*, Jeffrey B. Bonanno, Daniel J. Nilson, Alex J. Poppel, Lucas Dada, Sean M. Cahill, Juan Pablo Maianti, Seiya Kitamura, David Cowburn*, Steven C. Almo*
Biochemistry 2024, https://pubs.acs.org/doi/full/10.1021/acs.biochem.4c00331
tags: Biochemistry, cancer
Ultrapotent influenza hemagglutinin fusion inhibitors developed through SuFEx-enabled high-throughput medicinal chemistry
Seiya Kitamura*, Ting-Hui Lin, Chang-Chun David Lee, Akihiro Takamura, Rameshwar U. Kadam, Ding Zhang, Xueyong Zhu, Lucas Dada, Emiko Nagai, Wenli Yu, Yao Yao, K. Barry Sharpless, Ian A. Wilson*, and Dennis W. Wolan*
Proc. Nat. Acad. Sci. USA 2024, https://doi.org/10.1073/pnas.231067712
tags: High-throughput medicinal chemistry, infectious disease, SuFEx, inhibition
SuFEx-based chemical diversification for the systematic discovery of CRBN molecular glues
Trever R. Carter, Natalia Milosevich, Lucas Dada, James B. Shaum, K. Barry Sharpless, Seiya Kitamura*, Michael A. Erb*
Bioorganic & Medicinal Chemistry 2024, doi.org/10.1016/j.bmc.2024.117699
tags: High-throughput medicinal chemistry, molecular glues, SuFEx, cancer, inhibition
2023
PROTAC-mediated selective degradation of cytosolic soluble epoxide hydrolase enhances ER-stress reduction
, , , , Simone Sidoli, Jun Yang, , ,
ACS Chem. Biol. 2023, 18, 4, 884–896
tags: PROTACs, soluble epoxide hydrolase, ER stress
2022
The Development of the Bengamides as New Antibiotics against Drug-Resistant Bacteria
Cristina Porras-Alcalá,Federico Moya-Utrera,Miguel García-Castro,Antonio Sánchez-Ruiz,Juan Manuel López-Romero,María Soledad Pino-González,Amelia Díaz-Morilla,Seiya Kitamura,Dennis W. Wolan, José Prados,Consolación Melguizo,Iván Cheng-Sánchez, and Francisco Sarabia
https://www.mdpi.com/1660-3397/20/6/373
tags: Antibiotics, bacteria
Diversity oriented clicking delivers β-substituted alkenyl sulfonyl fluorides as covalent human neutrophil elastase inhibitors
Yunfei Cheng, Gencheng Li, Christopher J. Smedley, Marie-Claire Giel, Seiya Kitamura, Jordan L. Woehl, Giulia Bianco, Stefano Forli, Joshua A. Homer, John R. Cappiello, Dennis W. Wolan, John E. Moses, and K. Barry Sharpless
https://www.pnas.org/doi/10.1073/pnas.2208540119
tags: SuFEx, diversity-oriented click chemistry, protease, inhibition
PROTAC-mediated selective degradation of cytosolic soluble epoxide hydrolase enhances ER-stress reduction
, , , , , ,
tags: PROTACs, soluble epoxide hydrolase, ER stress
2021
Metabolomics activity screening of T cell–induced colitis reveals anti-inflammatory metabolites
Montenegro-Burke, J.R., Kok, B.K., Guijas, C., Domingo-Almenara, X., Moon, C., Galmozzi, A., Kitamura, S., Eckmann, L., Saez, E., Siuzdak, G.E., Wolan, D.W.
Science Signaling 2021 Vol 14, Issue 702
tags: microbiome, metabolomics, proteomics
A photoaffinity probe that targets folate-binding proteins.
Takamura, A., Thuy-Boun, P.S., Kitamura, S., Han, Z., Wolan, D.W.
Bioorg. Med. Chem. Lett. 2021 40, 127903
tags: microbiome, folate, ABPP, photo-activatable probe, proteomics
Chemical Inhibition of ENL/AF9 YEATS Domains in Acute Leukemia
Garnar-Wortzel, L.#, Bishop, T. # , Kitamura, S. #(co-first author), Milosevich, N., Asiaban, J., Zhang, X., Zheng, Q., Chen, E., Ramos, A., Ackerman, C., Hampton, E., Chatterjee, A., Young, T., Hull, M., Sharpless K.B., Cravatt, B., Wolan, D. W., Erb, M.*
ACS Cen. Sci. 2021, 7, 5, 815–830
tags: High-throughput medicinal chemistry, PROTAC, SuFEx, epigenetics, inhibition
2020
Synthetic elaboration of native DNA by RASS (SENDR).
Flood, D.T., Knouse, K.W., Vantourout, J.C., Kitamura, S., Sanchez, B.B., Sturgell, E.J., Chen, J.S., Wolan, D.W., Baran, P.S., Dawson, P.E.
ACS Cen. Sci. 2020 6, 1789-1799
tags: Bioconjugation, hNE inhibition
Discovery of small-molecule enzyme activators by activity-based protein profiling.
Kok, B.P., Ghimire, S., Kim, W., Chatterjee, S., Johns, T., Kitamura, S., Eberhardt, J., Ogaswara, D., Xu, J., Sukiasyan, A., Kim, S.M., Godio, C., Bittencourt, J.M., Cameron, M., Galmozzi, A., Forli, S, Wolan, D.W., Cravatt, B.F., Boger, D.L., Saez, E.
Nat. Chem. Biol. 2020 16, 997-1005
tags: activator, new modality, protease
An irreversible inhibitor to probe the role of Streptococcus pyogenes cysteine protease SpeB in evasion of host complement defenses.
Woehl, J.L.#, Kitamura, S.# (co-first author), Dillon, N., Han, Z., Edgar, L.J., Nizet, V., Wolan, D.W.
ACS Chem. Biol. 2020 15, 2060–2069
tags: microbiome, protease, ABPP, proteomics
An influenza A hemagglutinin small-molecule fusion inhibitor identified by a new high-throughput fluorescence polarization screen.
Yao, Y., Kadam, R.U., Lee, C.-C.D., Woehl, J.L., Chen, E., Wu, N.C., Zhu, X., Kitamura, S., Wilson, I.A., Wolan, D.W.
Proc. Nat. Acad. Sci. USA 2020 117, 18431-18438
tags: infectious diseases, inhibitor, screening
Sulfur(VI) fluoride exchange (SuFEx)-enabled high-throughput medicinal chemistry.
Kitamura, S., Zheng, Q., Woehl, J.L., Solania, A., Chen, E., Dillon, N., Hull, M.V., Kotaniguchi, M., Cappiello, J.R., Kitamura, S., Nizet, V., Sharpless, K.B., Wolan, D.W.
J. Am. Chem. Soc. 2020 142, 10899–10904
tags: High-throughput medicinal chemistry, SuFEx, protease
Diversity oriented clicking (DOC): Divergent synthesis of SuFExable pharmacophores from 2-substituted-alkynyl-1-sulfonyl fluoride (SASF) hubs.
Smedley, C.J., Li, G., Barrow, A.S., Gialelis, T.L., Giel, M.-C., Ottonello, A., Cheng, Y., Kitamura, S., Wolan, D.W., Sharpless, K.B., Moses, J.E.
Angew. Chem. Int. Ed. 2020 59, 12460-12469
tags: SuFEx, click chemistry, infectious diseases
Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria.
Bess, E.N., Bisanz, J.E., Yarza, F., Bustion, A., Rich, B.E., Li, X., Kitamura, S., Waligurski, E., Ang, Q.Y., Alba, D.L., Spanogiannopoulos, P., Nayfach, S., Koliwad, S.K., Wolan, D.W., Franke, A.A., Turnbaugh, P.J.
Nat. Microbiol. 2020 5, 56-66
tags: microbiome, metabolites, food
2019
SuFEx-enabled, agnostic discovery of covalent inhibitors of human neutrophil elastase.
Zheng, Q., Woehl, J.L., Kitamura S., Santos-Martins, D., Smedley, C.J., Lia, G., Forli, S., Moses, J.E., Wolan, D.W., Sharpless, K.B.
Proc. Nat. Acad. Sci. USA 2019 116, 18808-18814
tags: SuFEx, screening, hNE inhibition
Nanobody-based binding assay for the discovery of potent inhibitors of CFTR inhibitory factor (Cif)
Vasylieva, N., Kitamura, S., Dong, J., Barnych, B., Hvorecny, K.L., Madden, D.R., Gee, S.J., Wolan, D.W., Morisseau, C., Hammock, B.D.
Analyt. Chim. Acta 2019 1057, 106-113
tags: infectious diseases, screening, inhibitor
2018
Probing substrate recognition of bacterial lipoprotein signal peptidase using FRET reporters
Kitamura, S., Wolan, D.W.
FEBS Lett. 2018 592, 2289-2296
tags: infectious diseases, protease, inhibitor
Lipoprotein signal peptidase inhibitors with antibiotic properties identified through design of a robust in vitro HT platform
Kitamura, S., Owensby, A., Wall, D., Wolan, D.W.
Cell Chem. Biol. 2018 25, 301-308
tags: infectious diseases, protease, screening, inhibitor
2017
Occurrence of urea-based soluble epoxide hydrolase inhibitors from the plants in the order Brassicales
S Kitamura, C Morisseau, TR Harris, B Inceoglu, BD Hammock
PloS one 12 (5), e0176571
tags: food, inhibitor, hydrolase
Active-site flexibility and substrate specificity in a bacterial virulence factor: Crystallographic snapshots of an epoxide hydrolase
Kelli L Hvorecny, Christopher D Bahl, Seiya Kitamura, Kin Sing Stephen Lee, Bruce D Hammock, Christophe Morisseau, Dean R Madden
Structure 25 (5), 697-707. e4
tags: infectious diseases, structure
2016
Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa
S Kitamura, KL Hvorecny, J Niu, BD Hammock, DR Madden, C Morisseau
Journal of medicinal chemistry 59 (10), 4790-4799
tags: infectious diseases, structure, inhibitor, hydrolase
2015
Potent Natural Soluble Epoxide Hydrolase Inhibitors from Pentadiplandra brazzeana Baillon: Synthesis, Quantification, and Measurement of Biological Activities In Vitro and In Vivo
Seiya Kitamura, Christophe Morisseau, Bora Inceoglu, Shizuo G Kamita, Gina R De Nicola, Maximilienne Nyegue, Bruce D Hammock
PLoS One 10 (2), e0117438
tags: food, inhibitor, hydrolase
2014
